TURKISH JOURNAL OF ONCOLOGY
2020 , Vol 35 , Num 2
C Deletion in Exon 4 Codon 63 of p53 Gene in Turkish Patients with Oral Squamous Cell Carcinoma
1Department of Medical Biology, Amasya University, Faculty of Medicine, Amasya-Turkey2Department of Oncology, Gaziosmanpaşa University, Faculty of Medicine, Tokat-Turkey
3Department of Medical Genetics, Hitit University, Faculty of Medicine, Çorum-Turkey
4Department of Medical Biology, Gaziosmanpaşa University, Faculty of Medicine, Tokat-Turkey
5Department of Oral and Maxillofacial Surgery, Gaziosmanpaşa University, Faculty of Dentistry, Tokat-Turkey
6Department of Genetic, Ondokuz Mayıs University, Faculty of Veterinary, Samsun-Turkey DOI : 10.5505/tjo.2019.2092 OBJECTIVE
Oral squamous cell carcinoma (OSCC) is the most frequently seen oral malignancy and accounts for up to 80-90% of all malignant neoplasms that occurin the oral cavity. The p53 tumor suppressor gene plays a crucial role in the regulation of the cell cycle. Mutations of the p53 gene havean important role in OSCC carcinogenesis. In this study, we aimed to evaluate the C-deletion mutation in exon 4 codon 63 of p53 gene in Turkish patients with OSCC.
METHODS
A total of 60 subjects were enrolled in this study, 30 patients with a pathologic diagnosis of OSCC and
30 cases of age and sex-matched healthy controls. Genotyping was performed for all individuals using
polymerase chain reaction (PCR) analysis.
RESULTS
The findings showed that the distribution of p53 exon 4 codon 63 C-deletion was significantly different
between patient group and control group (p=0.000). It was detected that all patients had C-deletion
mutation in exon 4 codon 63 of p53.
CONCLUSION
Our results suggest that C-deletion in exon 4 codon 63 deletion of the p53 gene may play a role in the
pathogenesis of human OSCC in a Turkish cohort.