C Deletion in Exon 4 Codon 63 of p53 Gene in Turkish Patients with Oral Squamous Cell Carcinoma
Akın TEKCAN1,Özge GÜMÜŞAY2,Ayşe Feyda NURSAL3,Serbülent YİĞİT4,Serkan YILDIZ5,Mehmet Kemal TÜMER4
1Department of Medical Biology, Amasya University, Faculty of Medicine, Amasya-Turkey
2Department of Oncology, Gaziosmanpaşa University, Faculty of Medicine, Tokat-Turkey
3Department of Medical Genetics, Hitit University, Faculty of Medicine, Çorum-Turkey
4Department of Medical Biology, Gaziosmanpaşa University, Faculty of Medicine, Tokat-Turkey
5Department of Oral and Maxillofacial Surgery, Gaziosmanpaşa University, Faculty of Dentistry, Tokat-Turkey
6Department of Genetic, Ondokuz Mayıs University, Faculty of Veterinary, Samsun-Turkey
DOI : 10.5505/tjo.2019.2092 OBJECTIVE
Oral squamous cell carcinoma (OSCC) is the most frequently seen oral malignancy and accounts for up to 80-90% of all malignant neoplasms that occurin the oral cavity. The p53 tumor suppressor gene plays a crucial role in the regulation of the cell cycle. Mutations of the p53 gene havean important role in OSCC carcinogenesis. In this study, we aimed to evaluate the C-deletion mutation in exon 4 codon 63 of p53 gene in Turkish patients with OSCC.

A total of 60 subjects were enrolled in this study, 30 patients with a pathologic diagnosis of OSCC and 30 cases of age and sex-matched healthy controls. Genotyping was performed for all individuals using polymerase chain reaction (PCR) analysis.

The findings showed that the distribution of p53 exon 4 codon 63 C-deletion was significantly different between patient group and control group (p=0.000). It was detected that all patients had C-deletion mutation in exon 4 codon 63 of p53.

Our results suggest that C-deletion in exon 4 codon 63 deletion of the p53 gene may play a role in the pathogenesis of human OSCC in a Turkish cohort. Keywords : Deletion; oral squamous cell carcinoma; p53; PCR