TURKISH JOURNAL OF ONCOLOGY 2022 , Vol 37 , Num 4
Role of Interleukin-6 Gene Variants in the Development of Oral Squamous Cell Carcinoma
Özge GÜMÜŞAY1,Serbülent YİĞİT2,Ayşe Feyda NURSAL3,Akın TEKCAN4,Hasan DAGMURA5,Nilüfer KURUCA6
1Deparment of Oncology, Gaziosmanpasa University Faculty of Medicine, Tokat-Türkiye
2Deparment of Genetics, Ondokuz Mayis University Faculty of Veterinary, Samsun-Türkiye
3Deparment of Medical Genetics, Hitit University Faculty of Medicine, Çorum-Türkiye
4Deparment of Medical Biology, Amasya University Faculty of Medicine, Amasya-Türkiye
5Deparment of General Surgery, Bezmialem University Faculty of Medicine, İstanbul-Türkiye
6Deparment of Pathology, Ondokuz Mayis University Faculty of Veterinary, Samsun-Türkiye
DOI : 10.5505/tjo.2022.3624 OBJECTIVE
Oral squamous cell carcinoma (OSCC), with its low survival rates and increasing incidence, is due to various etiological factors including environmental, genetic, and epigenetic changes. Interleukin 6 (IL- 6) is a cytokine with both pro- and anti-inflammatory effects. Therefore, we investigated the possible association of the IL-6 gene variants with risk for OSCC in a Turkish cohort.

METHODS
This study included 42 patients with OSCC and 110 age-and gender-matched healthy controls. Three variants (-174G/C [rs1800795], -572G/C (rs1800796), and -597G/A [rs1800797]) in the IL-6 promoter region were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.

RESULTS
There was a significant difference in the genotype and allele frequencies of the IL-6 -174G/C variant between OSCC patients and controls. While the IL-6 -174G/C G/C genotype was higher in the patient group than in the control group, the G/G and C/C genotypes were lower in the patients compared to the control group (p=0.016, OR:0.653, 95% CI: 0.38-1.11). The genotype and allele distributions of -572G/C and -597G/A variants of the IL-6 gene were not statistically different between OSCC patients and the control group.

CONCLUSION
Our current investigation is focused on the role of variants of IL-6 gene on OSCC. To the best of our knowledge, this study is the first study to evaluate the genotype and allele frequencies of IL-6 -174G/C, -572G/C, and 597G/A variants in patients with OSCC in a Turkish population. The results support that the IL-6 -174G/C variant may play an important role in susceptibility to OSCC in our population. Keywords : Interleukin 6; oral squamous cell carcinoma; PCR-RFLP; variant